RBS
Part:BBa_K4046050:Design
Designed by: Andy Qiao Group: iGEM21_Duke (2021-10-19)
Kozak Sequence (Human RBS)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The start codon ATG is not included in this version of the Kozak sequence.
Source
The Kozak sequence was determined through analysis of various eukaryotic mRNAs.
References
Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125-48. doi: 10.1093/nar/15.20.8125. PMID: 3313277; PMCID: PMC306349.